Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach
نویسندگان
1 Developmental Biology Laboratory, School of Biology, College of Science, University of Tehran, Tehran, Iran
2 Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
3 Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
4 Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
5 Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
6 School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran
7 Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
8 Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
9 Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic)
10 Developmental Biology Laboratory, School of Biology, College of Science, University of Tehran, Tehran, Iran
11 Bioelectromagnetics Laboratory, School of Electrical and Computer Engineering, University of Tehran
12 INSF Chair of Computational Electromagnetics and Bio-electromagnetics, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran.
doi
10.22060/eej.2020.16230.5283چکیده
Weak microwave radiation (WMR) in our environment has raised health concerns in the public. Among those, communication frequencies are more than ever becoming widespread and their effects need thorough studies. A correct understanding of these effects in-vivo by in-vitro experiments shall preferentially use primary cells. In this study we compared non-modulated (CW) and modulated WMR exposure of biological cells in-vitro. Human ADMSC (Adipose-Derived Mesenchymal Stem Cells) were exposed to very weak non-thermal levels of microwave Electromagnetic fields at 1135 MHz ,SAR (Specific Absorption Rate) 0.002 W/kg (Watt per Kilogram) for 30 minutes daily for 4 days. A statistically significant decrease in proliferation rate of these stem cells was observed compared to the control group with no exposure. When amplitude-modulated exposure (15 Hz (Hertz) with a depth of 80%) was used with the same carrier frequency of 1135 MHz (Mega Hertz) and consistent average power, the cell numbers showed no statistically significant difference from the non-modulated exposure, but were nevertheless lower than the not-exposed control. The observed decrease in proliferation in response to -weak microwavefields supports the hypothesis that non-excitable cells, such as undifferentiated mesenchymal stem cells can interact with, and respond to weak electromagnetic radiation at communication frequencies. Possible mechanisms responsible for the observed results have been hypothesized and directions provided for future research.
