فهرست نشریات علمی دسترسی آزاد ایران

In vitro maturation is a critical step in in vitro fertilization, significantly impacting oocyte quality and subsequent embryonic development. This study examines how insulin-like growth factor (IGF)-1 supplementation in the maturation medium affects mitogen-activated protein kinase and maturation promoting factor expressions, as well as the amount of mitochondrial DNA copies in Kacang goat oocytes. Oocytes were collected from Kacang goat ovaries and matured in vitro with varying IGF-1 concentrations, including 0.00 (control), 50.00, 100, and 150 ng mL-1. Immuno-cytochemistry was used to assess mitogen-activated protein kinase and maturation promoting factor expressions, while quantitative polymerase chain reaction quantified mitochondrial DNA copy number. Results showed that the 100 ng mL-1 dose of IGF-1 group had substantially increased mitogen-activated protein kinase and maturation promoting factor expressions and also mitochondrial DNA copy numbers compared to the other groups. These findings suggest that IGF-1 supplementation with 100 ng mL-1 dose optimally enhances oocyte maturation by activating key signaling pathways and promoting mitochondrial replication. In conclusion, IGF-1 supplementation at 100 ng mL-1 is recommended to improve oocyte quality in Kacang goats, potentially enhancing in vitro fertilization outcomes.