فهرست نشریات علمی دسترسی آزاد ایران

Use of various nanoparticles and vitamins to strengthen the immune system of fish to improve growth and biochemical indice, and to mitigate the harmful effects of free radicals through enhanced antioxidant enzyme activity has become the subject of numerous studies. In this study, fish with an average weight of 55.20 ± 7.90 g, after a two-week acclimation period, were randomly distributed into 18 polyethylene 300-L tanks, with 25 fish per tank. The experiment consisted of six treatments with three replicates: Control, vitamin (Vit) C100, nano-selenium (N-Se)0.40, Vit C100 + N-Se0.10, Vit C200 + N-Se0.20, and Vit C400 + N-Se0.40. Blood samples were collected post-treatment for analysis of antioxidant enzyme activities, biochemical and hematological indices as well as post-handling stress. Results indicated significant differences in antioxidant enzyme activities among treatments. The highest catalase activity was observed in Vit C100 and Vit C100 + N-Se0.10 groups. Glutathione peroxidase activity was significantly higher in the control and Vit C200 + N-Se0.20 groups. Malondialdehyde was the highest in Vit C100 + N-Se0.10 group. Superoxide dismutase activity peaked in Vit C200 + N-Se0.20 group. Biochemical indices showed significant differences among treatments except for high-density lipoprotein. Hemoglobin and red blood cell counts were significantly different. The Vit C100 + N-Se0.10 group showed the highest serum cortisol and glucose levels post-handling stress. Overall, dietary N-Se and Vit C supplementation improved antioxidant, biochemical, hematological indices and stress resistance in rainbow trout.