Rotavirus infection in cattle
نویسندگان
1 Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia
2 Department of Mathematics, College of Science, Northern Border University, Arar, Saudi Arabia
3 Department of Pharmacy Practice, College of Pharmacy, Northern Border University, Arar, Saudi Arabia
4 Department of Mathematics, College of Science, Northern Border University, Arar, Saudi Arabia
5 Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia
6 Department of Biological Sciences, College of Science, Northern Border University, Arar, Saudi Arabia
7 Department of Pharmacology and Toxicology, College of Pharmacy, Northern Border University, Arar, Saudi Arabia
doi
10.30466/vrf.2025.2044037.4506چکیده
Rotavirus is one of the major causes of diarrhea in different animal species and has a bad economic impact due to the losses in neonates and productivity. To investigate the occurrence of this infection in bovine calves, three localities in Khartoum State, Sudan, were selected. A total of 200 fecal samples were collected from diarrheic calves; 100 from Khartoum and 50 from each of Khartoum Bahari and Omdurman provinces. Collected samples were screened for a group A rotavirus antigen using enzyme-linked immunosorbent assay (ELISA). Positive results were seen in 40.00% of samples; the highest prevalence of 42.00% was found in samples from Khartoum province. Five ELISA-positive samples were examined under electron microscope, and characteristic wheel-like appearance of rotavirus was visualized. Polyacrylamide gel electrophoresis was also applied on 15 of the positive samples; eight samples showed different polyacrylamide gel electrophoretic group A rotavirus long profile with different patterns. The results showed that the occurrence of rotavirus infection in cattle in Khartoum State is increasing.
