In-vitro evaluation and efficacy of bacteriophage isolated from commercial poultry farms against Salmonella typhimurium
نویسندگان
1 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
2 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
3 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
4 Veterinary Research Institute, Lahore, Pakistan
5 College of Earth and Environmental Sciences, University of the Punjab, Lahore, Pakistan
6 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
7 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
8 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
9 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
10 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
11 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
12 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
13 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
14 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
15 Institute of Microbiology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan
doi
10.30466/vrf.2024.2030436.4305چکیده
Avian Salmonellosis impacts the economy and public health, with chicken products being a major cause of gastroenteritis. Hygiene, immunization and medicines are all used as control techniques. Bacteriophages provide a safe, targeted alternative. In the present study in vitro evaluation of bacteriophages were done against Salmonella typhimurium. Lytic effect of bacteriophages isolated from poultry sludge was checked on culture of S. typhimurium. Stability study was checked at range of temperature and pH. The phages were stable at temperature (30.00 - 50.00 ˚C) and pH (5.00 - 9.00) where best activity was seen at 37.00 ˚C and pH 7.00. In vitro lytic activity was done at (optical density 600 nm) after exposure to bacterial host at different intervals. Multiplicity of Infection of 1.00 was used to check lytic activity of phages which indicated phages were potent enough to infect bacterial cells within their growth cycle. The percentage of unadsorbed phages was determined by bar chart analysis. The genome of three phages was treated with DNase I where they all were sensitive. Later the nucleic acid of phages was digested by restriction endonucleases (EcoRI and HindIII) both of the enzymes produced various restriction sites with different band. The present study proved that the application of bacteriophages in vitro into bacterial system i.e., S. typhimurium was an attractive method in diminishing infection in commercial poultry thus providing exceptional results that could be used on a large scale.
