Unlocking the beneficial effects of multi-enzyme cocktail Bacillus sonorensis BD92 on commercial broiler growth performance and intestinal histology
نویسندگان
1 Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
2 Department of Biosciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
3 National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Affiliated Institute of Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
4 National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Affiliated Institute of Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
5 Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan
6 Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Kyrgyz-Turkish Manas University, Bishkek, Kyrgyzstan
7 National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Affiliated Institute of Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
8 School of the Environment and Safety Engineering, Biofuels Institute, Jiangsu University, Zhenjiang, China
9 Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
10 National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Affiliated Institute of Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
11 National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan Affiliated Institute of Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan
12 Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye
doi
10.30466/vrf.2024.2025777.4221چکیده
Crude fiber (CF) is a vital component in poultry nutrition with a notable phytonutrient effectively indicating the presence of indigestible biomass in food due to the absence of digestive enzymes for CF in broilers. This study aimed to analyze the properties of a multi-enzyme cocktail (MEC) Bacillus sonorensis BD92 (BsBD92) comprised of xylanase, β-glucosidase, exo-glucanase, and endo-glucanase enzymes. Also, this study intended to look at the growth performance and intestinal histology of broilers in the starter and finisher phases by the addition of MEC BsBD92 to their diet. To evaluate the efficacy of MEC BsBD92, 140 one-day-old unsexed Cobb500 broiler chicks were randomly divided into seven groups receiving different diets. The characterization of exo-glucanase, xylanase, β-glucosidase, and endo-glucanase showed that their peak activities were observed at a temperature of 50.00 ˚C and a pH of 5.50. The 6.00% CF and 2.00 X MEC BsBD92 improved the intestinal morphology and feed conversion ratio, demonstrating a synergistic effect on growth performance. Whereas, increasing meat percentages to 61.06 and 65.09 g per 100 g body weight during the starter and finisher phases was also observed, respectively. The lipid profiles revealed significant variations in triglyceride and cholesterol levels. This study provides an innovative approach, considering not only lowering the feed cost using inexpensive fibrous feedstuffs but also improving the feed efficiency through supplementation of MEC BsBD92.
