Association of miR-200a and miR-205 Expression with Epithelial-Mesenchymal Transition in Colorectal Cancer Cells
نویسندگان
1 Department of Biology, Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran
2 Basic and Molecular Epidemiology of Gastrointestinal Disorder Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Science, Tehran, Iran
3 Department of Biology, Faculty of Science, Islamic Azad University, Science and Research Branch, Tehran, Iran.
4 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences. Tehran, Iran
5 Department of Genetics, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
doi
10.22037/aab.v11i3.31476چکیده
Abstract Introduction: MicroRNAs play a critical role in EMT regulation through tightly regulating the transcription factors. EMT (Epithelial-Mesenchymal Transition) in the colorectal cancer cell (CRC) is a highly controlled mechanism, contributing to the development of progressive cancer. Throughout this research, miR-200a/205 was focused as a component implicated throughout regulating the EMT process in colorectal cancer cells. In this sense, the induction of the EMT process was made using colorectal cancer cell lines. Materials and Methods: The mRNA levels of E-cadherin, Vimentin, β-catenin, Zeb1, and Snail were determined using real time-PCR for characterization of the EMT process. True real time-PCR was conducted for evaluating the alteration amount of microRNAs. Results: The findings of this study verified the in-vitro EMT model being developed. The in vitro analysis revealed a negative correlation between the Zeb1 and Snail miR-200a and miR205 (P=0.001) (P=0.0001). The results of miR-200a and miR205 are regulated down in vitro. Conclusion: miR-200a and miR205 may be used as candidates in further research to prevent colorectal cancer's invasive properties via the EMT process.
