Luteal activity of Abadeh ecotype does in summer and winter and the effect of a single dose of kisspeptin-10 injection on luteinizing hormone secretion in the anestrus does
نویسندگان
1 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
2 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
3 Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
5 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
6 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
7 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
8 Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
9 Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences and Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
10 Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
doi
چکیده
The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P4) concentration in 10 goats in summer (n = 6) and winter (n = 4) were measured every other day. Moreover, in summer group, a male teaser goat was left in the herd on days of sampling for one hour. Goats with P4 concentration ≥1 ng mL-1, at least two consecutive measurements, were considered with luteal activity. In the second study, the anestrous phase was confirmed by P4 measurement 20 and 10 days before the kisspeptin injection in five female Abadeh ecotype goats (4 to 5 years old). The goats were given a single IV injection of saline (2 mL) as control group and the same goats (1 hr after the last blood sampling) were given kisspeptin (1 μg kg-1) as treatment group. The blood samples were collected at –60, –40, –20 and 0 min (before injection), and 10, 20, 30, 40, 50, 60, 80, 100, 120 and 140 min after the injection and LH concentration were measured. A single IV injection of 1 µg kg-1 of kisspeptin-10 did not stimulate the release of LH in female anestrous goats. In summer, in the presence of teaser goat, luteal activity was seen in all goats. In the absence of male goat in winter, some goats showed luteal activity and others showed anestrus.
