Cloning of Oct3/4 gene in embryonic stem cells
نویسندگان
1 Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran
2 Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran
3 Department of Genetics, Islamic Azad University, Science and Research Branch, Tehran
4 National Research Centre of Genetic Engineering and Biotechnology, Tehran
5 Department of Haematology, Tarbiat Modares University, Tehran
6 Department of Genetics, Islamic Azad University, Science and Research Branch, Tehran
doi
10.22037/jps.v2i1.2147چکیده
Embryonic stem cells (ESCs) are pluripotent, self-renewing cells. These cells can be used in applications such as cell therapy, drug discovery, disease modelling, and the study of cellular differentiation. In this experimental study embryonic stem cells cultured in the laboratory and were amplified. Total RNA was extracted from cells and converted to cDNA. The replication factor Oct3/4 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into the pTZ57R/T vector. Legated product had been transformed into susceptible bacteria and transformed bacteria were screened on a selective medium. Plasmids extracted from bacteria and enzyme digestion to confirm the sequencing was performed. Results of enzyme digestion were sequenced. Cloned gene can prepare a gene cassette to produce stem cells from somatic cell.
