The Combined Effects of Mesenchymal Stem Cell Conditioned Media and Low-Level Laser on Stereological and Biomechanical Parameter in Hypothyroidism Rat Model
نویسندگان
1 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Department of Biological Sciences,Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, Iran
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5 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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doi
20867چکیده
Introduction: Many studies have shown the positive effect of laser radiation and application of the mesenchymal stem cells (MSCs) and their secretion in stimulating bone regeneration. The aim of this study was determining effects of MSC conditioned media (CM) and low-level laser (LLL) on healing bone defects in the hypothyroid male rat.Methods: We assigned 30 male Wistar rats randomly to 3 groups: control, hypothyroidism, CM+LLL. Four weeks after surgery, the right tibia was removed. Biomechanical examination and histological examinations were performed immediately.Results: Our results showed significant increase in bending stiffness (116.09 ± 18.49), maximum force (65.41 ± 8.16), stress high load (23.30 ± 7.14), energy absorption (34.57 ± 4.10), trabecular bone volume (1.34 ± 0.38) and the number of osteocyte, osteoblast, and osteoclast (12.77 ± 0.54, 6.19 ± 0.80, 1.12 ± 0.16 respectively) in osteotomy site in the LLL + CM group compared to the hypothyroidism group (P < 0.05).Conclusion: The results indicated that using the LLL + CM may improve fracture regeneration and it may hasten bone healing in the hypothyroid rat.
