Determination of 5-Hydroxymethyl-2-furaldehyde of Crude and Processed Fructus Corni in Freely Moving Rats Using In Vivo Microdialysis Sampling and Liquid Chromatography: Determination of 5-hydroxymethyl-2-furaldehyde by HPLC
نویسندگان
1 Research Center of TCM Processing Technology, Zhejiang Chinese Medical University， Hangzhou 310053, PR China
2 Research Center of TCM Processing Technology, Zhejiang Chinese Medical University， Hangzhou 310053, PR China
3 Jiangsu Key Laboratory of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210029, PR China
4 Department of Pharmaceutics, Zhejiang Chinese Medical University, Hangzhou 310053, P.R. China
5 Research Center of TCM Processing Technology, Zhejiang Chinese Medical University， Hangzhou 310053, PR China
6 Research Center of TCM Processing Technology, Zhejiang Chinese Medical University， Hangzhou 310053, PR China
doi
10.22037/ijps.v7.41355چکیده
The purpose of this study was to develop a sensitive and fast microdialysis coupled with high-performance liquid chromatographic (HPLC) method for determination of 5-hydroxymethyl-2-furaldehyde (5-HMF) in free-moving rats after i.g. administration of the aqueous extract of crud Fructus corni and its processed products of jiuzheng pin (JZP). The concentration of 5-HMF in free-moving rats after i.g. administration of the water extract of Fructus corni and JZP was analyzed by microdialysis coupled with HPLC. Results demonstrated that the concentration of 5-HMF in microdialysate was 1.4951μg/l, but higher in rat microdialysate after i.g. administration of the aqueous extract of JZP (5.2662 μg/l). In conclusion, this method is proved to be rapid，accurate and simple. Real-time in vivo monitoring the concentration of 5-HMF provides the theoretical basis for further explaining the processing mechanism of F. corni.
