Development and Application of a Validated LiquidChromatography-Mass Spectrometry Method fortheDetermination of Dexchlorpheniramine Maleate in Human Plasma: Determination of dexchlorpheniramine

نویسندگان
Aravindaraj Joghee Raju ID 1
Gopinath Ram ID 2
Suresh Bhojraj ID 3
Rajan Sekar ID 4
Mahesh Kumar Siddaiah ID 5
Nanjan Moola Joghee ID 6

1 CADRAT, JSS College of Pharmacy

2 CADRAT, JSS College of Pharmacy

3 Principal, JSS College ofPharmacy, Rock lands, Ootacamund, India

4 CADRAT, JSS College of Pharmacy

5 TIFAC CORE, JSS College of Pharmacy

6 TIFAC CORE, JSS College of Pharmacy

doi
10.22037/ijps.v3.41015
چکیده

Aconvenient liquid chromatographic-single Quadrupole mass spectrometric(LC-MS) method was developed and validated for dexchlorpheniramine maleate (INNname: chlorphenamine) determination in human plasma. The need for just a singleliquid-liquid extraction with ethyl acetate and being highly sensitive were theadvantages of this method. The linearity was also excellent over the range of 1 to150 ng.ml-1of dexchlorpheniramine maleate concentration. The method wasstatistically validated for its selectivity, linearity, precision and robustness. This methodwas successfully applied to the analysis of chlorpheniramine maleate in clinicalstudies.

کلیدواژه‌ها
Bioequivalence, Dexchlorpheniramine maleate, LC-MS