Determination of Vitamin C in Small Volumes of Blood by HPLC/EC: Determination of vitamin C in blood
نویسندگان
1 Department of Pharmaceutical Sciences, Strathclyde Institute for Biomedical Sciences, 27 Taylor St., Glasgow G4 0NR, U.K.
2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
4 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
doi
10.22037/ijps.v1.39436چکیده
A sensitive procedure for determining total vitamin C (ascorbic acid + dehydrate ascorbic Acid) in a blood drop from a finger prick, before and after the administration of a vitamin C tablet is described. Analysis was carried out by high performance liquid chromatography with electrochemical detection (HPLC/EC).Measurements were taken one hour, two hours and six hours after the administration of a 500 mg vitamin C tablet. D-Isoascorbic acid was used as the internalstandard and analysis was carried out using two C-18 columns connected in series and a phosphate buffer mobile phase. Dehydroascorbic acid in the samples was converted to ascorbic acid by incubation with DL-homocysteine for 30 minutes. The level of vitamin C in blood reached a maximum concentration after twohours.
