The Effect of Fe203 Nanoparticles on Sperm Parameters and the Expression of Mitochondrial Apoptotic Pathway Genes in Rats With Scrotal Hyperthermia
نویسندگان
1 Department of Cell Biology and Genetics, Bushehr Branch, Islamic Azad University, Bushehr, Iran.
2 Farhikhtegan Medical Convergence Science Research Center, Farhikhtegan Hospital, Tehran Medical sciences, Islamic Azad University, Tehran, Iran.
3 Department of genetics; Islamic Azad University; Tehran Medical Branch; Tehran, Iran.
4
5 Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
doi
10.22037/aab.v13i.36734چکیده
Introduction: Hyperthermia can cause infertility in men following an increase in testiculartemperature. Oxidative stress has been found to be one of its major causes. In the present study,the effects of iron superoxide nanoparticles on the expression of Bcl-2 and Bax family geneswere studied.Materials and Methods: A total of 48 adult rats were purchased from the Pasteur Instituteof Iran. The rats were later divided into 4 groups: control group, control group receivingsuperoxide nanoparticles (Fe2O3), hyperthermia group, h’:yperthermia group receivingsuperoxide nanoparticles (Fe2O3). After RNA extraction, evaluating the sperm parameters andthe expression of Bax and Bcl-2 genes was examined using RT-PCR technique.Results: Exposure to iron superoxide nanoparticles (Fe2O3) decreased sperm parameters,increased proapoptotic BAX gene and decreased expression of BCL2 anti-apoptotic gene.Conclusion: Exposure to nanoparticles by reducing sperm parameters and increasing apoptosishas a negative effect on fertility. The association between infertility and testicular hyperthermiais becoming increasingly apparent; administration of iron superoxide (Fe2O3) nanoparticlescan have significant effects on male infertility. Moreover, green synthesis of nanoparticles isalso recommended in this field.
