فهرست نشریات علمی دسترسی آزاد ایران

Introduction: Flask-adherent Splenocytes (SACs) fulfill antigen-presenting cell requirementsof acquired immune responses. This study was done to evaluate the efficacy of newimmunotherapy against breast cancer made by SACs pulsed with the extract of heated 4T1cells and Lactobacillus casei, as a probiotic.Materials and Methods: Mammary carcinoma was induced by injection of 4T1 cell line inthe flank of female Balb/c mice. The first SACs therapy was started on day 11 after tumorinduction when all animals had developed a palpable tumor. SACs therapy was done twice ata 10-day interval.Results: Mice with mammary tumors received SACs pulsed with combined heated 4T1 cellsand L. casei determined a more desirable survival curve and a slower rate of tumor developmentcompared to the other groups. At least 20% of the group receiving combined immunotherapywere alive by day 58. Those mice receiving SACs pulsed with the Lysate of heated tumorcells died by day 45.The maximum survival of other mice was up to 38 days after tumorinduction. Moreover, SAC pulsed with combined agents significantly amplified the secretionof Interferon-γ (IFN-γ), and conversely reduced the secretion of Transforming growth factor-β(TGF-β) and Interleukin 4 (IL-4) in the splenocyte population compared to splenocytes fromother groups. Combined immunotherapy increased the expression of p53 and caspase 3 genesand reduced the exertion of BCL2 more than other immunotherapy protocols.Conclusion: Immunotherapy with SACs pulsed with heated 4T1 cells and L. casei promotesbeneficial outcomes in the mouse model of breast cancer.