فهرست نشریات علمی دسترسی آزاد ایران

Background and objectives: This article delve into the valuable insights provided by molecular markers, particularly Matrix Metalloproteinase 9 (MMP-9), in the context of pulpal diagnosis. It emphasizes the challenges associated with current diagnostic tools and advocates for enhanced methods to ensure a reliable diagnosis. Elevated MMP-9 levels emerge as potential biomarkers not only for pulpal diseases but also for periodontal and cardiovascular conditions. The exploration extends to molecular alterations and diagnostic biomarkers in gingival crevicular fluid, shedding light on promising avenues for dental diagnosis and customized treatment approaches. Materials and methods: Gingival crevicular fluid (GCF) samples were collected from 56 eligible patients using medium-sized paper points. A second set of GCF samples was collected two weeks post- root canal treatment. Normal samples were obtained from patients referred for routine dental check-ups. GCF samples were transferred to sampling tubes with Tris HCL pH = 7.5 buffer. MMP-9 levels were measured using ELISA. Results: Statistical analysis revealed a significant decrease in MMP-9 levels in GCF after root canal treatment for both symptomatic and asymptomatic irreversible pulpitis (P-value < 0.05). The study identifies an increase in MMP-9 during pulp inflammation. Subsequently, MMP-9 levels decrease in the gingival crevicular fluid after root canal treatment, indicating the resolution of inflammation. Conclusion: Precision in evaluating and measuring enzymes associated with pulp tissue destruction in the local gingival crevicular fluid is achievable through a precise method. The examination of MMP-9 in gingival crevicular fluid can serve as a valuable tool for monitoring inflammatory activity and assessing the success of treating teeth with symptomatic and asymptomatic irreversible pulpitis.