Cytoplasmic and membranous CD24 marker expression has indirect correlation with cAMP/cGMP ratio
نویسندگان
1 Shahid Beheshti Medical University, Tehran, Iran
2 Shahid Beheshti Medical University, Tehran, Iran
3 Shahid Beheshti University, Tehran, Iran
4 Shahid Beheshti Medical University, Tehran, Iran
5 Shahid Beheshti Medical University, Tehran, Iran
6 Shahid Beheshti Medical University, Tehran, Iran
doi
10.22037/nbm.v1i3.5429چکیده
Background- CD24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. Our aim was clarify correlation between CD24 expression and cAMP:cGMP ratio in murine colorectal cancer cell line (CT26) after using cholera toxin.Materials and Methods- The CT26 cells were cultured in microtubes for assaying cAMP and cGMP; also the cells were cultured in flasks for assaying cytoplasmic and membranous CD24 expression. The Real-Time PCR was done for cDNA that was synthesized from CT26 cells’ mRNA. Also, expression CD24 marker of cells was determined by Anti-CD24 antibody and Goat Anti-Rabbit IgG-FITC (flow cytometry).Results- The cholera toxin grew cAMP:cGMP ratio and it influenced cytoplasmic and membrane CD24 expression.Conclusion- There are indirect correlation between cAMP:cGMP ratio and CD24 expression.
