فهرست نشریات علمی دسترسی آزاد ایران

Staphylococcus aureus is one of the most prevalent microorganisms in both medical and veterinary field. This bacterium often could infect in areas of damaged skin, such as abrasions and open wound. This research aims to detect the common type and antibiotic resistant pattern of isolated S. aureus in wound specimens. One hundred and fifty wound swabs were collected from human, sheep and goat (50 equal samples) in West and East Azarbaijan provinces (Iran). All swabs' samples were transferred to the laboratory in peptone water broth near ice box. The samples were cultured aerobically on sheep blood agar medium at 37 °C for 24 h. Then polymerase chain reaction was used for amplification 16S rRNA and mecA genes to identify the S. aureus and methicillin-resistant S. aureus (MRSA). Then antimicrobial susceptibility test was done for antibiotic resistant patterns configuration using some antibiotic discs. S. aureus was isolated from 19 wound samples and 6 isolates were identified as methicillin-resistant S. aureus. The higher and lower isolate was belonged to human (52 %) and goat (8%), also 40 % of sheep wound samples were infected by S. aureus. No animal wound samples had MRSA while six human specimens were infected by MRSA. All isolates were sensitive against cefepime, imipenem and carbapenem and the higher resistances were seen against penicillin, vancomycin and cefazolin. It's concluded that MRSA is common in infected human wound and today new generation of cephalosporins are useful for treatment of many multidrug resistant bacteria.