Dose‑dependent efficacy of antioxidant nanoparticles on red blood cells storage
نویسندگان
1 Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran, Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran, Department of Oncology‑Pathology, Immune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska University Hospital Solna and Karolinska Institute, Stockholm, Sweden
2 Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
3 Department of Biotechnology , School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4 Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
5 Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
6 Department of Pathobiology and Laboratory Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
7 Department of Immunology, School of Medicine, Iranian Blood Transfusion Research Center, Tarbiat Modares University, Tehran, Iran
8 Department of Pathobiology and Laboratory Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
doi
چکیده
BACKGROUND: Transfusion of healthy red blood cells (RBCs) after storage is important. One ofthe storage lesions on blood bags is oxidative stress. One way to prevent increased oxidative stressis to use antioxidant nanoparticles (NPs). Superoxide dismutase (SOD) and catalase (CAT) playan important role in antioxidant defense on RBC. poly lactic-co-glycolic acid (PLGA) is a nontoxicbiodegradable polymer that is approved by the Food and Drug Administration for drug delivery. Thisstudy aimed to assess dose-dependent efficacy of SOD-CAT-polyethylene glycol -PLGA on RBCsstorage.MATERIALS AND METHODS: Using a descriptive study, during 1 month, twenty donors fromBojnourd Blood Donation Center were selected. NPs with different concentrations were injectedinto the satellite bags after directing blood to them. On target days, experiments were performed onthe samples taken. Electrospray was employed to prepare SOD-CAT-PLGA NPs. Twenty packedRBCs were isolated from the whole blood bags by the mechanical method, and certain amount ofproduct was transferred to the satellite bags. On days 1, 7, 14, 21, 28, and 35, bags were sampled.Malondialdehyde (MDA), prooxidant-antioxidant balance (PAB), and Annexin V were performed onthe samples taken. The repeated measures analysis with the help of SPSS software version 20was performed on samples.RESULTS: MDA increased in both groups. The maximum increase in test group was seen inconcentration 12 mg (MDA Day 14, test [1.93 ± 0.3], [P MDA < 0.001]). Maximum increase in PABwas seen in concentration 12 mg (from 444 ± 1.7 to 563 ± 2.5) (P PAB = 0.000). Furthermore,PS expression increased in the concentration of 12 mg greater than other concentration inconsecutive (from 5.00 ± 0.8 to 22.26 ± 1.7, [P < 0.001]).CONCLUSION: Evaluation of dose dependency showed that different concentrations of antioxidantNPs affect RBC. This effect can be changed oxidative stress and apoptosis. Using both changes toevaluate functional and toxicity can be helpful.
