Transplantation of Cardiogenic Pre-Differentiated Autologous Adipose-Derived Mesenchymal Stem Cells Induced by Mechanical Loading Improves Cardiac Function Following Acute Myocardial Infarction in Rabbit Model
نویسندگان
1 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
2 Cardiovascular Lab, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
3 Cardiovascular Lab, Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
4 National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
5 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
6 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
7 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
8 National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
9 National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
10 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
11 Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
12 Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
doi
چکیده
Objective- Investigate myocardial performance after autologous adipose-derived (ASCs) mesenchymal stem cell differentiated under equiaxial cyclic strain, transplantation in rabbits with acute myocardial infarction (AMI).Design- Prospective, randomized experimental studyAnimals- 20 New Zealand White rabbits (2-3 kg)Procedure- ASCs were studied in four distinct groups of mechanical (ADM), chemical (ADC), undifferentiated (AD) and control (C) groups. According to this categorization, the cells were exposed to cyclic mechanical loading or 5-azacytidine as the chemical factor. 10 6 ASC cells were transplanted intramyocardially in rabbits with AMI (Acute Myocardial Infarction). Echocardiographic study was used to evaluate effects of cells on cardiac function.Results- Left ventricle ejection fraction (LVEF) was significantly increased in the ADM (mechanically-differentiated adipose-derived mesenchymal stem cell) group at 2 months follow-up. Fractional shortening (FS) also showed a similar pattern as LVEF and increased in ADM group in compare to control and AD (undifferentiated adipose-derived mesenchymal stem cell) group.Conclusion and clinical prevalence- The results indicate that intramyocardial transplantation of mechanically-differentiated ASCs improves cardiac function of ischemic myocardium. Transplantation of mechanically-differentiated ASCs for myocardial regeneration may become the future therapy for acute myocardial infarction.
