Comparison of Two Multiplex PCR Systems for Meat Species Authentication

نویسندگان

1 Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Environment, Tehran North Branch, Islamic Azad University, Tehran, Iran

4 Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

5 Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

6 Department of Pathophysiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

doi
10.18502/jfqhc.6.1.453
چکیده

Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication.  Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward ...