Potential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
نویسندگان
1 Institute of Biological Sciences, Faculty of Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
2 Institute of Biological Sciences, Faculty of Sciences, University of Malaya, 50603 Kuala Lumpur, Malaysia
doi
10.29252/jfqhc.5.3.94چکیده
Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated ...
