Assessment of Anticancer Effect of Rhodium Nanoparticle-based Photodynamic Therapy via Protein-Protein Interaction Network Analysis: Anticancer effect of rhodium nanoparticle-based photodynamic therapy
نویسندگان
1 Laser Application in Medical Sciences Center, Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
2 Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
3 Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
4 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 1-Hematology-Oncology and Stem Cell Transplantation Research Center, Research Institute for Oncology, Hematology, and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran 2-Iranian Cancer Control Center (MACSA), Tehran, Iran
doi
50791چکیده
Introduction: Photodynamic therapy, as an efficient and safe method, has attracted the attention of experts. This therapeutic method is based on the application of photosensitizers and light radiation. This study was designed to assess the possible molecular mechanism of rhodium nanoparticle-based photodynamic therapy through protein-protein interaction (PPI) network analysis of proteomic data from the literature.Methods: Proteomic data about rhodium nanoparticle-based photodynamic therapy effect on the HeLa cell line proteome were retrieved from the literature and were included in the CluePedia application of Cytoscape software to create a directed PPI network. The network was analyzed, and the crucial targeted proteins were identified and compared with genes in GeneCards for “HeLa cell line” and “cervical cancer”. The common gene and protein wereselected and discussed.Results: A directed PPI network of 105 proteins was formed. Six sub-networks were selected for further investigation. Comparison of the PPI data and the genes from the GeneCards database ledto the introduction of HLA-B, CYCS, CD44, HSPB1, and RBBP4 as the critical targeted proteins by the applied treatment.Conclusion: In conclusion, a sub-network including HLA-B, CYCS, CD44, and HSPB1 and another sub-network containing RBBP4 and its neighbors were highlighted as the core of molecular effects of the applied rhodium nanoparticle-based photodynamic therapy.
