Effect of Photobiomodulation Therapy with Three Wavelengths of Diode Laser on Proliferation and Survival of Stem Cells Derived from Impacted Third Molar Follicles: Photobiomodulation & stem cell proliferation from third molar follicles
نویسندگان
1 Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran/Department of Oral and Maxillofacial Surgery, Shariati Hospital, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
4 Private Practitioner, Researcher at Tehran University of Medical Sciences, Tehran, Iran
5 Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran / Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 Private Practitioner, Researcher at Tehran University of Medical Sciences, Tehran, Iran
doi
48609چکیده
Introduction: Increased tissue regeneration is facilitated by mesenchymal stem cells (MSCs), with photobiomodulation (PBM) effectively promoting their proliferation. This study examines the impact of PBM on human third molar follicle stem cells using three diode lasers, addressing the need for further investigation regarding its parameters. Methods: The third molar follicle stem cells of four consented patients were removed after surgical extraction of third molars. These cells were then exposed to laser radiation with wavelengths of 660 (energy density: 3 and 5.1 J/cm2), 808 (energy density: 3 and 5 J/cm2), and 980 (energy density: 3.2 and 5/2 J/cm2) nanometers at time intervals of 24, 48, and 72 hours. The proliferation and viability of the cells were determined using the MTT test. The data were analyzed through one-way ANOVA followed by Tukey’s HSD post hoc test, utilizing SPSS software version 22, with a significance threshold of 0.05. Results: The highest average number of human third molar follicle stem cells was obtained at 660 nanometers and an energy density of 5.1 J/cm2 in 24 hours. This specific laser protocol significantly outperformed other conditions in promoting cellular proliferation. Conclusion: PBM at the 660-nm wavelength administered with a dose of 5.1 J/cm² and a 24-hour incubation period appears to be the most effective condition for enhancing the proliferation and survival of human third molar follicle stem cells. These findings provide a potential basis for standardized PBM protocols in regenerative dentistry.
