Introducing Aurora Kinase A as a Gene Target Against Photoaging: A Network Analysis: Aurora kinase A as a gene target against photoaging
نویسندگان
1 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 5 Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
4 Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
5 1Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 1. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran 2.Iranian Cancer Control Center (MACSA), Tehran, Iran
7 Celiac Disease and Gluten Related Disorders Research Center, Research Institute for Gastroenterology and Liver Disease, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
8 Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
9 1. Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2. Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung
doi
47335چکیده
Introduction: The solar radiation spectrum, which reaches the Earth’s surface, spans from infrared to ultraviolet. In the present study, human skin response to solar-stimulation radiation was assessed via directed protein-protein interaction (PPI) network analysis. Methods: Data were extracted from the Gene Expression Omnibus (GEO) database. The gene expression profiles of human skin after exposure to 100J/M2 versus the control were selected from GSE22083. The data were evaluated via box plot analysis and Uniform Manifold Approximation and Projection (UMAP) plot assessment. The differentially expressed genes (DEGs) were visualized via a Venn diagram. After cleaning the data, the queried DEGs were assessed via the directed PPI network by the application of the CluePedia plugin of Cytoscape software. The critical DEGs were pointed out based on out-degree value. Key actor genes were searched in GeneCards to find a suitable description of them. Results: Among 1482 significant DEGs, AURKA, CENPA, PP2R1B, UBE2O, RPA3, YKT6, SMARCA5, and SMAD3 were identified and highlighted as the critical actor genes in response to solar-stimulated radiation in human skin. Conclusion: AURKA appeared as the top-ranked DEGs in response to solar radiation in the human skin. Based on the findings, AURKA was pointed out as a suitable target against photoaging. The relationship between solar-stimulated radiation and photoaging, cancer promotion, innate immune system, DNA replication, repair of UV damage-induced, vesicular trafficking between the Golgi apparatus and the endoplasmic reticulum, exocytosis of neurotransmitter, exosome production, autophagy, maintenance of nucleosome spacing, and process of progressive fibrosis were established
