The Application of Photobiomodulation and Stem Cells Seeded on the Scaffold Accelerates the Wound Healing Process in Mice: Stem cells seeded on scaffold plus photobiomodulation on wounds healing process
نویسندگان
1 Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
2 Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
3 Department of Anatomical Sciences and Cognitive Neuroscience at the Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4 Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
5 Department of Anatomical Sciences and Cognitive Neuroscience at the Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
6 Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
7 Price Institute of Surgical Research at the University of Louisville and Noveratech LLC of Louisville in Louisville, KY, USA
8 Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
9 Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
10 Department of Tissue Engineering & Regenerative Medicine, Iran University of Medical Sciences, Hemmat Highway, Tehran, Iran
11 Xi’an jiaotong University School of Stomatology, Xi’an, Shaanxi Province, China
12 Tissue Engineering Department, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
13 Department of Biology and Anatomical Sciences at Shahid Beheshti University of Medical Sciences (SBMU), Tehran, Iran
14 1-Department of Tissue Engineering & Regenerative Medicine, Iran University of Medical Sciences, Hemmat Highway, Tehran, Iran 2-Price Institute of Surgical Research at the University of Louisville and Noveratech LLC of Louisville in Louisville, KY, USA
doi
44789چکیده
Introduction: The purpose of this research was to test the impact of seeding a hydrogel chitosan scaffold (HCS) with human adipose-derived stem cells (hADSCs) under the influence of photobiomodulation (PBM) on the remodeling step of the wound repairing process in mice.Methods: Thirty mice were randomly assigned to five groups (n=6 per group ): The control group (group 1) consisted of mice without any intervention. In group 2, an HCS was implanted into the wound. In group 3, a combination of HCS+hADSC was inserted into the wound. In group 4, an HCS was inserted into the wound and PBM was applied. In group 5, a combination of HCS+hADSCs was inserted into the wound, followed by PBM treatment.Results: Improvements in the injury closing rate (WCR) and microbial flora were observed in all groups. However, the highest WCRs were observed in groups 5, 4, 3, and 2 (all P values were 0.000). Groups 3-5 showed increased wound strength compared to groups 1 and 2, with group 2 demonstrating better results than group 1 (P values ranged from 0.000 to 0.013). Although groups s 3-5 showed increases in certain stereological elements compared to group s 1 and 2, group 2 exhibited superior results in comparison with group 1 (P values ranged from 0.000 to 0.049).Conclusion: The joined use of HCS+hADSCs+PBM significantly accelerated the wound healing process during the maturation phase in healthy mice. This approach demonstrated superior wound healing compared to the use of HCS alone, hADSCs+HCS, or PBM+HCS. The findings suggest an additive effect when HCS+hADSCs+PBM are combined.
