Photoinactivation Effects of Curcumin, Nano-curcumin, and Erythrosine on Planktonic and Biofilm Cultures of Streptococcus mutans: PDT effects of curcumin and erythrosine on S. mutans
نویسندگان
1 Dental Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2 Student Research Committee, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
3 Dental Materials Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4 Student Research Committee, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran
5 Department of Restorative, Preventive, and Pediatric Dentistry, University of Bern, Bern, Switzerland
6 Antimicrobial Resistance Research Center, Department of Microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
7 1. Department of Medical Laboratory Sciences, School of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran 2. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
8 1. Radiation Sciences Research Center, AJA University of Medical Sciences, Tehran, Iran 2. International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research, Network (USERN), Tehran, Iran
doi
42832چکیده
Introduction: This in vitro study was conducted to assess the phototoxic effects of curcumin, nanocurcumin, and erythrosine on the viability of Streptococcus mutans (S. mutans) in suspension and biofilm forms.Methods: Various concentrations of curcumin (1.5 g/L, 3 g/L), nano-curcumin (3 g/L), and erythrosine (100 μM/L, 250 μM/L) were examined for their impact on planktonic and biofilm cultures of S. mutans, either individually or in conjunction with light irradiation (photodynamic therapy or PDT). A blue light-emitting diode (LED) with a central wavelength of 450 nm served as the light source. The results were compared to 0.12% chlorhexidine digluconate (CHX) as the positive control, and a solution containing neither a photosensitizer (PS) nor a light source as the negative control group. The dependent variable was the number of viable microorganisms per experiment (CFU/mL).Results: Antimicrobial PDT caused a significant reduction in the viability of S. mutans in bothplanktonic and biofilm forms, compared to the negative control group (P<0.05). The highest cellkilling was observed in PDT groups with curcumin 3 g/L or erythrosine 250 μmol/L, although thedifference with PDT groups using curcumin 1.5 g/L or erythrosine 100 μmol/L was not significant(P>0.05). Antimicrobial treatments were more effective against planktonic S. mutans than thebiofilm form.Conclusion: PDT with either curcumin 1.5 g/L or erythrosine 100 μmol/L may be suggested as an alternative to CHX to inactivate the bacteria in dental plaque or deep cavities. Nanocurcumin, at the selected concentration, exhibited lower efficacy in killing S. mutans compared to Curcumin or erythrosine.
