Photobiomodulation Therapy in the Proliferation and Differentiation of Human Umbilical Cord Mesenchymal Stem Cells: An In Vitro Study
نویسندگان
1 Department of Restorative Dentistry and EndodonticsSchool of DentistryUniversity of PernambucoBrazil
2 Department of Restorative Dentistry and Endodontics, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, Brazil
3 Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, Recife, PE, Brazil
4 Department of Restorative Dentistry and Endodontics, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, Brazil
5 Center of Biophotonics, School of Dentistry, Universidade Federal da Bahia, Salvador, BA, Brazil
6 Department of Restorative Dentistry and Endodontics, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, Brazil
7 3Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
8 Department of Restorative Dentistry and Endodontics, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, Brazil
9 Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, Recife, PE, Brazil
10 Department of Restorative Dentistry and Endodontics, School of Dentistry, Universidade de Pernambuco, Camaragibe, PE, Brazil
11 Department of Biophysics and Radiobiology, Universidade Federal de Pernambuco, Recife, PE, Brazil
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27911چکیده
Introduction: Since photobiomodulation therapy (PBMT) favors in vitro mesenchymal stem cell (MSC) preconditioning before MSC transplantation, increasing the proliferation of these cells without molecular injuries by conserving their characteristics, in the present in vitro study we analyzed the effect of PBMT on the proliferation and osteogenic differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs).Methods: Irradiation with an InGaAIP Laser (660 nm, 10 mW, 2.5 J/cm2, 0.08 cm2 spot size, and 10 s) was carried out. The cells were divided into four groups: CONTROL [cells grown in Dulbecco’s Modified Eagle Medium (DMEM)], OSTEO (cells grown in an osteogenic medium); PBMT (cells grown in DMEM+PBMT), and OSTEO+PBMT (cells grown in an osteogenic medium-plus PBMT). The cell proliferation curve was obtained over periods of 24, 48 and 72 hours using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Osteogenic differentiation was analyzed by the formation of calcium nodules over periods of 7, 14 and 21 days. Morphometric analysis was performed to quantify the total area of nodular calcification.Results: The highest cell proliferation and cell differentiation occurred in the OSTEO+PBMT group, followed by the PBMT, OSTEO, and CONTROL groups respectively, at the observed times (P < 0.05).Conclusion: PBMT enhanced the osteogenic proliferation and the differentiation of hUCMSCs during the periods tested, without causing damage to the cells and preserving their specific characteristics, a fact that may represent an innovative pretreatment in the application of stem cells.
