Assessment of the Microbiome Role in Skin Protection Against UV Irradiation Via Network Analysis

نویسندگان
Mohammad Hossein Heidari ID 1
Mohammad Rostami-Nejad ID 2
Mostafa Rezaei-Tavirani ID 3
Mona Zamanian Azodi ID 4
Mohammad Reza Razzaghi ID 5
Alireza Akbarzadeh Baghban ID 6
Alireza Zali ID 7
Alireza Ahmadzadeh ID 8

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doi
29631
چکیده

Introduction: Diverse microbiotas which have some contributions to gene expression reside in human skin. To identify the protective role of the skin microbiome against UV exposure, protein-protein interaction (PPI) network analysis is used to assess gene expression alteration.Methods: A microarray dataset, GEO accession number GSE117359, was considered in this respect. Differentially expressed genes (DEGs) in the germ-free (GF) and specific pathogen-free (SPF) groups are analyzed by GEO2R. The top significant DEGs were assigned for network analysis via Cytoscape 3.7.2 and its applications.Results: A total of 28 genes were identified as significant DEGs and the centrality analysis of the network indicated that only one of the seven hub-bottlenecks was from queried genes. The gene ontology analysis of Il6, Cxcl2, Cxcl1, TNF, Il10, Cxcl10, and Mmp9 showed that the crucial genes were highly enriched in the immune system.Conclusion: The skin microbiome plays a significant role in the protection of the skin against UV irradiation and the role of TNF and IL6 is prominent in this regard.

کلیدواژه‌ها
Microbiome, UV radiation, Gene expression, Protein-protein interaction network