Assessment of Sterility and Residual Solvents Agents in Antler-derived and Bovine Xenograft Bone Substitutes: An In-vitro Study
نویسندگان
1 Professor Dept. of Periodontics, School of Dentistry & Dental Research Center, Research Institute of Dental Sciences Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2 Assistant Professor, Dept. of Periodontics, School of Dentistry & Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3 Assistant Professor, Dept. of Periodontics, Faculty of Dentistry & Tissue Engineering, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4 Student Research Committee, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Professor Dept. of Periodontics, School of Dentistry & Dental Research Center, Research Institute of Dental Sciences Shahid Beheshti University of Medical Sciences, Tehran, Iran.
6 Associate Professor, Dept. of Periodontics, School of Dentistry & Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
7 Student Research Committee, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran
doi
10.22037/jds.v40i1.40075چکیده
Objectives This study aimed to assess and compare the results of sterility and residual solvent testing in a newly developed antler-derived xenograft versus a bovine-derived xenograft. Methods First, test and control samples were prepared using thermal and chemical procedures, involving immersion in deionized water for 24 hours, drying, boiling in sterile water, chemical treatment with chloroform and methanol, and heating at 650°C in a furnace. Next, they were sterilized via gamma radiation at 25 kGy. The sterility test was then performed based on the ISO 11737-2:2019 standard, using the direct inoculation method. Finally, residual solvent testing was carried out via gas chromatography-mass spectrometry. Results The sterility test showed no evidence of bacterial or fungal growth in any of the samples during 14 days of incubation. Also, residual solvent testing indicated no sign of residual solvents in the samples. Conclusion Antler-derived xenograft was safe to use in terms of the sterility and removal of residual solvents. Further studies should be carried out regarding other important laboratory tests as well as the animal and clinical studies.
