Lethality Assay of Radiopharmaceutical bis-Thiosemicarbazones Using Brine Shrimp (Artemia salina) Test: Toxicity of bis-thiosemicarbazones
نویسندگان
1 Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran.
2 Faculty of Pharmacy, Azad Islamic University, Tehran, Iran.
3 Research Institute for Nuclear Medicine, Tehran University of Medical Sciences, Tehran, Iran
4 Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran.
5 Radiopharmaceutical Research and Development Lab (RRDL), Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran.
doi
10.22037/ijps.v8.40942چکیده
In the present study, aqueous solutions of some copper-complexing ligands were screened for their cytotoxicity using brine shrimp lethality test. Among the ligands tested, diacetyl-bis(N4- methylthiosemicarbazones) (ATSM) proved to be the most safe and non-toxic compound (2% lethality at 10 ppm), while pyruvaldehyde Bis(N4-methyl)thiosemicarbazone (PTSM) was shown to possess low toxicity (7% lethality at 10 ppm) and glyoxal-bis-thiosemicarbazone (GTS) proved to be a toxic compound even at 1 ppm (20% lethality). An interesting structure-toxicity relationship was observed for the ligands based on their water solubility leading to more toxicity which can be related to polysaccharide crustae of the shrimps. Considering the 1-10 ppm to be the maximum possible concentration of the ligands in the finalpharmaceutical samples, the safety of these ligands are ATSM>PTSM>GTS.
