Development and Validation of a Rapid Derivative Spectrophotometric Method for Determination of Tropicamide in Eye Drops: Determination of tropicamide in eye drops
نویسندگان
1 Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran
2 Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran
3 Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran
4 Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), Iran
doi
10.22037/ijps.v6.41269چکیده
Tropicamide is an antimuscarinic agent used as eye drops for refractive examinations. The aim of this study was to develop a simple and suitable analytical method for determination of tropicamide in eye drops in the presence of excipients. A zero-crossing third and fourth derivative spectrophotometric method was described for determination of tropicamide in eye drops. The measurements were carried outat wavelengths of 263.8 and 255.4 nm for third- and fourth-derivative, respectively. The method was found to be linear (r2> 0.999) in the range of 10-100 µg/ml for tropicamide in the presence of excipients. The limit of determination was 10 mg/ml for tropicamide. The method was successfully applied for determination of tropicamide in eye drops without any interference from excipients or need to prior separation before analysis.
