فهرست نشریات علمی دسترسی آزاد ایران

CONTEXT: Multidose injection vials (MDVs) are prone to bacterial contamination, and their use hasbeen reported to be a potential source of infections.AIMS: The aim of this study was to evaluate the knowledge and common practises of nursing staffregarding the use of MDVs and its microbial contamination rate.SETTINGS AND DESIGN: A pilot study was conducted in a super‑specialty hospital from June toDecember 2016.SUBJECTS AND METHODS: Information about knowledge and common practises of 100 nursingstaff posted in various Intensive Care Units (ICUs) with respect to the usage of single and MDVs,respectively, was obtained and assessed. About 40 in‑use multidose injection vials containingsome remnants were collected from different ICUs. The volume of 1 ml content of each of thesevials was inoculated into a tube containing 15 ml thioglycolate broth and incubated at 37°C for10 days. The broth was visually examined every day and subcultured onto blood, chocolate, andSabouraud Dextrose agar plates on alternate days within 10 days or any time that the appearanceseemed turbid. The microbial isolates thus obtained were identified using standard guidelinesand recorded.STATISTICAL ANALYSIS USED: Descriptive statistics were used.RESULTS: The study group members had sufficient knowledge about various aspects of handlingsingle and MDVs, respectively, such as hand hygiene, disinfection, checking of vial labels, and expirydate. Low hand hygiene compliance rate of 55% was observed in all ICUs visited during this study.The contamination rate of MDVs injection vials was 25% with Coagulase‑negative Staphylococcusspp. being the most common isolate.CONCLUSIONS: The use of MDVs is associated with the risk of contamination and nosocomialoutbreaks of life‑threatening bloodstream infections. Healthcare professionals must strictlyadhere to basic infection control practises as per standard guidelines to minimize the incidence ofhospital‑acquired infections.