Estimating the carbohydrate content of various forms of tobacco by phenol‑sulfuric acid method
نویسندگان
1 Department of Public Health Dentistry, Ajeenkya DY Patil Dental School, Pune, Maharashtra, India
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doi
چکیده
BACKGROUND: Due to consumption of various forms of tobacco in large amounts by Indianpopulation, it has become a cause of concern for major oral diseases. In 2008, the WHO namedtobacco as the world’s single greatest cause of preventable death. It is also known that certain amountof carbohydrates are incorporated in processed tobacco to make it acceptable for consumption. Thus,its role in oral diseases becomes an important question at this point of time. Through this study, it isattempted to find out the carbohydrate content of various forms of tobacco by phenol‑sulfuric acidmethod.MATERIALS AND METHODS: Tobacco products selected for the study were Nandi hookahtambakhu (A), photo brand budhaa Punjabi snuff (B), Miraj (C), Gai‑chhap tambakhu (D),Hanuman‑chhap Pandharpuri tambakhu (E), and Hathi‑chhap Bidi (F). The samples were decodedand transported to laboratory and tested at various concentrations by phenol‑sulfuric acid methodfollowed by ultraviolet spectrophotometry to determine their absorbance.RESULTS: The present study showed Hathi‑chhap bidi/sample F had a maximum absorbance (1.995)at 10 µg/ml which is a smoking form of tobacco followed by rest all smokeless forms of tobacco,i.e. sample C (0.452), sample B (0.253), sample D (0.077), sample E (−0.018), and sample A (−0.127),respectively.CONCLUSION: As the concentration of tobacco sample increases, their absorbance increaseswhich in turn is suggestive of increase in its carbohydrate concentration. Carbohydrates in the formof sugars, either inherently present or added in it during manufacturing can serve as a risk factor forhigher incidence of dental caries.