Synergistic effects of oxypeucedanin and temozolomide on viability, proliferation, apoptosis, and migration of T98G malignant glioblastoma cells
نویسندگان
1 Student Research Committee, Birjand University of Medical Sciences, Birjand, 9717853577, Iran
2 Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3 3- Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4 Department of medical laboratory science, Mashhad branch, Islamic Azad University, Mashhad, Iran
5 Imam reza hospital
6 - Student Research Committee, Birjand University of Medical Sciences, Birjand, 9717853577, Iran
7 Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
8 Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
doi
10.22038/ajp.2025.26224چکیده
Objective: Glioblastoma multiforme (GBM), an aggressive primary brain tumor, distinguished by an invasive growth pattern and resistance to current therapeutic strategy. This study investigates the potential of Oxypeucedanin (OP) as a natural compound to induce apoptosis and inhibit proliferation in T98G GBM cells, either alone or in combination with Temozolomide (TMZ).Materials and Methods: T98G cells were exposed to OP and TMZ individually and in combination. Then, cell viability (MTT assay), cell proliferation (using trypan blue), mRNA expression (qRTPCR), Cell cycle and apoptosis (flow cytometry), and migration (wound healing assay) were evaluated.Results: The viability assays revealed that both OP and less potentially TMZ decreased cell viability in a time- and dosedependent manner. Notably, the combination of OP and TMZ demonstrated synergistic effects, substantially enhancing apoptosis rates while reducing proliferation, as evidenced by reduced cell growth rates and altered cell cycle distribution towards G2/M arrest. Additionally, gene expression analysis indicated increased Bax/Bcl2 ratios and decreased Ki-67 levels, suggesting enhanced apoptotic susceptibility and lowered proliferation capacity. Furthermore, the wound healing assay confirmed reduced migration in T98G cells, particularly in the combination treatment group.Conclusion: This study suggests the potential of OP as a complementary therapeutic agent alongside TMZ for GBM treatment.
