Extracts of Apium graveolens (Celery) attenuate hepato-renal injury induced by chronic administration of gentamicin in mice through activation of Nrf2-antioxidant signaling pathways
نویسندگان
1 Pharmacology Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
2 Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé 1, PO Box 812, Yaoundé, Cameroon
3 Pharmacology Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
4 Pharmacology Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
5 Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaounde 1, Cameroon
6 Medical Research and Applied Biochemistry Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
7 Anatomy Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
8 Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaounde 1, Cameroon
9 Pharmacology Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
10 Higher Institute of Health Sciences, Université des Montagnes, P.O. Box 208, Bangangté, Cameroon
11 Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaounde 1, Cameroon
12 Pharmacology Laboratory, Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, PO Box 63, Buea, Cameroon
doi
10.22038/ajp.2024.25338چکیده
Objective: This study aimed at investigating the protective effect of extracts from Apium graveolens against gentamicin-induced hepato-renal toxicity.Materials and Methods: The aqueous and hydro-ethanolic extracts of A. graveolens designated respectively as WAG and HAG were tested for their in vitro antioxidant activities. Then, their cytoprotective effects were assessed against gentamicin-induced cytotoxicity in primary mouse hepatocytes. Finally, mice were administered with gentamicin (20 mg/kg) and co-treated with HAG for 14 days, and histopathology, biochemical and molecular parameters related to gentamicin-induced toxicity were evaluated.Results: HAG exhibited outstanding chemical antioxidant activities and preserved hepatocytes from gentamicin-induced cytotoxicity. HAG relieved liver and kidney histopathological and biochemical changes, and enhanced the mRNA level of Nrf2 and its target gene HO-1 in gentamicin-intoxicated mice.Conclusion: HAG attenuates hepato-renal injuries induced by 14-days administration of gentamicin in mice through the activation of Nrf2-antioxidant signaling pathways.
