فهرست نشریات علمی دسترسی آزاد ایران

Background :   Water is an important source of numerous infectious diseases in humans. The most important indicator bacteria include Escherichia coli and total coliform species. Clostridium perfringens is a general indicator of treatment efficiency. Methods: Diagnosing microbial contamination of water by culture method, in addition to high cost, has low speed and many limitations. The PCR (Polymerase Chain Reaction) method is a promising alternative for simultaneous detection of these bacteria. In this research, we developed multiplex PCR for detection of lacZ , uidA and plc genes as an indicator of the presence of total coliforms, Escherichia coli and Clostridium perfringens . In the following, 33 samples of water and wastewater from Razavi Khorasan province were tested using standard culture methods and this multiplex PCR for comparison. Results: Multiplex PCR was not only confirmed by cultural results but also has more accuracy and sharpness. The results of the validation tests showed that our multiplex PCR method had a significant advantage over the conventional culture method. Conclusion :   The developed multiplex PCR method demonstrated superior accuracy and efficiency compared to traditional culture techniques, offering a reliable alternative for rapid detection of waterborne pathogens.